Composite

Part:BBa_K3396006

Designed by: Yuxin Liu   Group: iGEM20_NUDT_CHINA   (2020-10-26)
Revision as of 12:41, 26 October 2020 by FloraLiu (Talk | contribs)


GFPnano-Coh2

To make sure that GFPnano-GFP can connect with TRIM21-DocS, we designed the part which bind Coh2 and GFPnano.


Usage and Biology

Coh2 is a kind of protein which bind with DocS spontaneously. GFPnano is the nanobody of GFP, a green fluorescent protein, which can be observed easily. This part can target the protein and connect with TRIM21-DocS and lead to the degradation in a Trim-Away alike method. Here is the mechanism of the recombined TRIM21-DocS:

1. The GFPnano tagged with Coh2 combines with targeted protein.

2. TRIM21-DocS connect Coh2-GFPnano-target through the DocS-Coh2 interaction.

3. The targeted protein is degraded by ubiquitin-proteasome system recruited by TRIM21.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 35


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Categories
Parameters
None