Composite

Part:BBa_K3396006

Designed by: Yuxin Liu   Group: iGEM20_NUDT_CHINA   (2020-10-26)


GFPnano-Coh2

To make sure that the target GFP protein can connect with TRIM21-DocS, we designed the part which contains Coh2 and GFPnano.


Usage and Biology

Coh2 is a kind of protein which bind with DocS constitutively. GFPnano is the nanobody of GFP, the reporter protein, which can be observed easily. This part can target the GFP protein and connect with TRIM21-DocS and lead to the degradation in a Trim-Away alike method.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 35


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Categories
Parameters
None