Composite

Part:BBa_K3606035:Design

Designed by: Zhenru Zhang   Group: iGEM20_Fudan   (2020-10-23)
Revision as of 07:44, 23 October 2020 by Registry (Talk | contribs)

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PhoA-CaAP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 138
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We linked PhoA to the N-terminal of CAAP for its secretion into the extracellular space.


Source

The sequence of PhoA is obtained from BBa_K808028 while the sequence of CAAP is artificially designed based on oligopeptides obtained from tilapia bone collagen hydrolysate, etc.

References