Composite
Part:BBa_K3606035:Design
Designed by: Zhenru Zhang Group: iGEM20_Fudan (2020-10-23)
PhoA-CaAP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 138
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The composite part PhoA_CaAP is a combination of signal peptide PhoA and CaAP(Calcium Absorption Peptide). The sequence of PhoA is obtained from BBa_K808028 with no further codon optimization. Then we fused PhoA (BBa_K808028) to the N-terminal of CaAP(BBa_K3606000) for its secretion into the extracellular space.
Source
The sequence of PhoA is obtained from BBa_K808028 while the sequence of CAAP is artificially designed based on oligopeptides obtained from tilapia bone collagen hydrolysate, etc.