Coding

Part:BBa_K3468085:Design

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-20)
Revision as of 16:11, 22 October 2020 by YuanWang (Talk | contribs)


PETase S142P


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NONE Reading the literature found that proline can be introduced in the flexible loop to improve the thermal stability of the protein. Ser142 is in the loop area of ​​PETase, and the b-factor value is higher than the average.

Source

Ideonalla sakaiensis

References