Coding

Part:BBa_K3468085:Design

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-20)


PETase S142P


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Reading the literature found that proline can be introduced in the flexible loop to improve the thermal stability of the protein. Ser142 is in the loop area of ​​PETase, and the b-factor value is higher than the average.

Source

Ideonalla sakaiensis

References