Coding
Knt-GFP

Part:BBa_K3427001

Designed by: Clementine Sejotte and Florent Poubanne   Group: iGEM20_GO_Paris-Saclay   (2020-10-20)
Revision as of 14:41, 20 October 2020 by Mathieunairabeze (Talk | contribs)


knt-gfp entangled CDSs for aminoglycoside resistance and GFP


A gene coding for the spectinomycin resistance that we named Knt and a gene coding for Green Fluorecent Protein ewas entangled.

The gene was cloned in the plasmid pBAD24-MoClo under the control of an arabinose-inducible promoter. Bacteria carrying this plasmid exhibited a green fluorescence when grown in the presence of arabinose

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 784
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 784
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 784
    Illegal BamHI site found at 378
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 784
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 784
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None