Coding

Part:BBa_K3611000

Designed by: Peixuan Zheng   Group: iGEM20_NEU_CHINA   (2020-10-20)
Revision as of 12:01, 20 October 2020 by Arcdia (Talk | contribs)


The coding gene of ACE2-PmrB recombinant protein. 

 This part is the coding gene of the recombinant protein of human angiotensin-converting enzyme 2 (ACE2) and bacteria PmrB protein of PmrA/PmrB tow component system.
 We replaced the original PmrB' s Fe (III) sensitive domains Trp34 to Glu64 with the core binding domain of ACE2 protein Thr20 to Asn90 in order to receive stimulation from the S protein of SARS-CoV-2. Upon sensing S protein of SARS-CoV-2, the sensor kinase PmrB autophosphorylates a highly conserved histidine residue and subsequently transfers the phosphoryl group to a conserved aspartate residue in its cognate response regulator PmrA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 534
    Illegal AgeI site found at 121
    Illegal AgeI site found at 127
    Illegal AgeI site found at 175
    Illegal AgeI site found at 741
    Illegal AgeI site found at 766
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None