Coding

Part:BBa_K3611000

Designed by: Peixuan Zheng   Group: iGEM20_NEU_CHINA   (2020-10-20)


The coding gene of ACE2-PmrB recombinant protein.


This part is the coding gene of the recombinant protein of human angiotensin-converting enzyme 2 (ACE2) and bacteria PmrB protein of PmrA/PmrB two component system.

We replaced the original PmrB' s Fe (III) sensitive domains Trp34 to Glu64 with the core binding domain of ACE2 protein Thr20 to Asn90 in order to receive stimulation from the S protein of SARS-CoV-2. Upon sensing S protein of SARS-CoV-2, the sensor kinase PmrB autophosphorylates a highly conserved histidine residue and subsequently transfers the phosphoryl group to a conserved aspartate residue in its cognate response regulator PmrA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1156
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None