Part:BBa_K3427000
Heat-stable fluorescent protein YFP_LOV
The thermostable flavin-based fluorescent protein stable_YFP_LOV was originally described as a domain of a bigger protein with histidine kinase activity from the Chloroflexus aggregans, a thermophilic phototrophic bacterium (A thermostable flavin-based fluorescent protein from Chloroflexus aggregans: a framework for ultra-high resolution structural studies Nazarenko et al. 2019, Photochem Photobiol Sci, doi: 10.1039/c9pp00067d). The sequence predicted to confer fluorescence was cloned along with a short fragment encoding a tag of 6 histidines. The His-tag could be useful in the future for purification purposes. The gene was cloned in the plasmid pBAD24-MoClo under the control of an arabinose-inducible promoter. Bacteria carrying this plasmid exhibited a green fluorescence when grown in the presence of arabinose.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
//function/reporter/fluorescence
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