Composite

Part:BBa_K3431048

Designed by: Huan-Jui Chang, Cheng-Ruei Yang, Hung-Yu Chen   Group: iGEM20_CSMU_Taiwan   (2020-10-17)
Revision as of 03:48, 18 October 2020 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


T7 promoter + RBS + mRFP + T7 terminator

This is a monomeric RFP generating device compatible with T7 RNA Polymerase in PURExpress in vitro protein synthesis kit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 763
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 604
    Illegal AgeI site found at 716
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None