Coding

Part:BBa_K3419001:Design

Designed by: Leo Song   Group: iGEM20_Cornell   (2020-10-17)
Revision as of 19:31, 17 October 2020 by Registry (Talk | contribs)

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Tricosanthin


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 409
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 409
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 409
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 409
    Illegal NgoMIV site found at 456
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was originally found in the amino acid sequence form. Thus, that sequence was reverse translated into a DNA sequence, and a stop codon of TGA was added at the end.


Source

Trichosanthin is from the genome of Trichosanthes kirilowii, also known as the Chinese cucumber.

References