Coding

Part:BBa_K3419001:Design

Designed by: Leo Song   Group: iGEM20_Cornell   (2020-10-17)


Tricosanthin


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 409
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 409
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 409
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 409
    Illegal NgoMIV site found at 456
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was originally found in the amino acid sequence form. Thus, that sequence was reverse translated into a DNA sequence, and a stop codon of TGA was added at the end.


Source

Trichosanthin is from the genome of Trichosanthes kirilowii, also known as the Chinese cucumber. The amino acid sequence was found at: https://www.uniprot.org/uniprot/P09989

References