Translational_Unit

Part:BBa_K3521005

Designed by: Kong Yangyang   Group: iGEM20_SHSID   (2020-10-14)
Revision as of 16:37, 14 October 2020 by Registry (Talk | contribs)

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pT7-FnCpf1-His tag

This part is the core element of FnCpf1 protein expression. The method of use is to add IPTG to induce the expression of FnCpf1 protein in E. coli BL21 (DE3). FnCpf1 protein was purified in vitro with a nickel column, and then used to target and cleave specific DNA sequences mediated by gRNA to make it fluorescent.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1784
    Illegal NheI site found at 3504
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3565
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None