Coding
Malat1

Part:BBa_K3504005

Designed by: Ahmed Wael   Group: iGEM20_AFCM-Egypt   (2020-09-19)
Revision as of 07:39, 11 October 2020 by AhmedAdel01 (Talk | contribs) (→‎Usage)


Malat1 triple helix

Part Description

Malat1 is an RNA triple helix (triplex) structure from the MALAT1 long noncoding RNA that has been used in several synthetic biology studies to stabilize engineered transcripts.

Usage

MALAT1 is a sequence that would stabilize the transcript and allow for translation after removal of special degradation tags. A RNA triple helix (triplex) structure from the MALAT1 long noncoding RNA has been utilized in a few manufactured science studies for stabilization of engineered transcripts. Overall this is an ON-switch that displays a promising scope of 166-fold, which is unique as an RNA ON-switch motif for transgene regulation.

Characterization

Improvements

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 38
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 38
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 38
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 38
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None