Part:BBa_K3504005
Malat1 triple helix
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Part Description
Malat1 is an RNA triple helix (triplex) structure from the MALAT1 long noncoding RNA that has been used in several synthetic biology studies to stabilize engineered transcripts.
Usage
MALAT1 is a sequence that would stabilize the transcript and allow for translation after removal of special degradation tags. A RNA triple helix (triplex) structure from the MALAT1 long noncoding RNA has been utilized in a few manufactured science studies for stabilization of engineered transcripts. Overall this is an ON-switch that displays a promising scope of 166-fold, which is unique as an RNA ON-switch motif for transgene regulation.(1)
References
1-DiAndreth, B., Wauford, N., Hu, E., Palacios, S., & Weiss, R. (2019, January 01). PERSIST: A programmable RNA regulation platform using CRISPR endoRNases. Retrieved October 26, 2020, from https://www.biorxiv.org/content/10.1101/2019.12.15.867150v1.full Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 38
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 38
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 38
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 38
- 1000COMPATIBLE WITH RFC[1000]
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