Reporter

Part:BBa_K3380153:Design

Designed by: Alexandru Popov   Group: iGEM20_Edinburgh   (2020-10-08)
Revision as of 11:10, 10 October 2020 by Alexandru popov (Talk | contribs) (→‎Source)


Broccoli fluorescent RNA aptamer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

Filonov et al. 2014 used directed evolution to obtain the 49 nucleotides Broccoli fluorescent RNA aptamer.

We acquired as sequences from Sigma-Aldrich

References

Filonov, G.S., Moon, J.D., Svensen, N. and Jaffrey, S.R., 2014. Broccoli: rapid selection of an RNA mimic of green fluorescent protein by fluorescence-based selection and directed evolution. Journal of the American Chemical Society, 136(46), pp.16299-16308.