Coding

Part:BBa_K3505003

Designed by: Asteria Tsapadikou , Magdalini Koroxenidou, Foteini Papadaki   Group: iGEM20_Thessaly   (2020-10-09)
Revision as of 15:04, 9 October 2020 by Asteriat (Talk | contribs)

LacI-Lac repressor GB compatible with B3-B5

Coding region for the LacI protein without an RBS.

Usage and Biology

The lac inhibitor and its operator is usually use for gene regulation and together they form a genetic circuit which called : the lac operon. In bacteria ,in abundance of lactose , lactose binds to lacI and the lac operon is activated. In synthetic biology , as an inducer we use IPTG, which doesn’t degraded in the cell , instead of lactose . IPTG is a molecular mimic of a lactose metabolite, which activates transcription of the lac operon. The lacI has the ability to bind to the lac operator (BBa_R0010 and BBa_R0011 hybrid regulator) inhibiting the expression of the desirable transcription unit.

Design Notes

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Sequence and Features No part name specified with partinfo tag.

[edit]
Categories
//cds/transcriptionalregulator/repressor
Parameters
biologyE. coli
proteinLacI