Coding

Part:BBa_K103004

Designed by: Michael Lower   Group: iGEM08_Warsaw   (2008-09-14)
Revision as of 00:59, 28 October 2008 by Smaegol (Talk | contribs)

protein ZSPA-1

Artificially created strong interactor of B domain of Staphylococcal protein A (BBa_K103003)[http://www.ncbi.nlm.nih.gov/pubmed/15238637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]. Codon usage optimized for expresion in E.coli. It autoaggregates easily even in fusion with soluble protein. Preferentially binds B domain of Staphylococcal protein A (BBa_K103003).

  • ZSPA-1-tagged proteins can be easily purified (more 99% of the protein is present in postsonication debris)
  • We have used ZSPA-1 protein fusions with BBa_K103019 or BBa_K103020 as a prey in our 'hunter and prey' selection system
  • This part uses our NdeI/SacI/BamHI fusion cloning substandard
Z spa.jpg

Source: [http://www.pdb.org/pdb/explore.do?structureId=2B88 PDB]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/ligand
Parameters
None