Part:BBa_K103004

protein Z_SPA-1

Artificially created strong interactor of B domain of Staphylococcal protein A ( BBa_K103003). Codon usage optimized for expresion in E.coli. It autoaggregates easily even in fusion with soluble protein. Preferentially binds B domain of Staphylococcal protein A [http://www.ncbi.nlm.nih.gov/pubmed/15238637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]. ZSPA-1-tagged proteins can be easily purified (more 99% of the protein is present in postsonication debris) We have used ZSPA-1 protein fusions with BBa_K103019 or BBa_K103020 as a prey in our 'hunter and prey' selection system This part uses our NdeI/SacI/BamHI fusion cloning substandard

Source: [http://www.pdb.org/pdb/explore.do?structureId=2B88 PDB]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]