Coding

Part:BBa_K3610011:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-09-08)
Revision as of 23:48, 3 October 2020 by Jtrott (Talk | contribs) (→‎Design Notes)


CORE ectodomain - codon optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 918
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1764


Design Notes

The high GC content of C. reinhardtii leads to a strong bias towards certain codons in C. reinhardtii. The codon frequency for each amino acid was chosen in a way that it is either the preferred codon or at least not lower than 5%.

Source

Cyril Zipfel Lab at UZH, Switzerland Codon otpimized with SnapGene

References