Coding
Part:BBa_K3610011:Design
Designed by: Jonas Sebastian Trottmann Group: iGEM20_UZurich (2020-09-08)
CORE ectodomain - codon optimized for C. reinhardtii
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 918
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1764
Design Notes
The high GC content of C. reinhardtii leads to a strong bias towards certain codons in C. reinhardtii. The codon frequency for each amino acid was chosen in a way that it is either the preferred codon or at least not lower than 5%.
Source
Cyril Zipfel Lab at UZH, Switzerland Codon otpimized with SnapGene
References
Wang, Lei; Albert, Markus; Einig, Elias; Fürst, Ursula; Krust, Damaris; Felix, Georg (2016): The pattern-recognition receptor CORE of Solanaceae detects bacterial cold-shock protein. In: Nature plants 2, S. 16185. DOI: 10.1038/nplants.2016.185.