Coding

Part:BBa_K3610009:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-09-08)
Revision as of 19:01, 3 October 2020 by Jtrott (Talk | contribs) (Design Notes)


FLS2 ectodomain from S. lycopersicum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 847
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2086
    Illegal AgeI site found at 472
    Illegal AgeI site found at 760
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The juxtamembrane domain must be included in the sequence to ensure proper dimerization with the coreceptor BAK1.

Source

This part is derived from the S. lycopersicum genome.

References