Part:BBa_K3610009:Design

FLS2 ectodomain from S. lycopersicum

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 847
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2086
  Illegal AgeI site found at 472
  Illegal AgeI site found at 760
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The juxtamembrane domain must be included in the sequence to ensure proper dimerization with the coreceptor BAK1.

Source

This part is derived from the S. lycopersicum genome.

References

Chinchilla, Delphine; Zipfel, Cyril; Robatzek, Silke; Kemmerling, Birgit; Nürnberger, Thorsten; Jones, Jonathan D. G. et al. (2007): A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. In: Nature 448 (7152), S. 497–500. DOI: 10.1038/nature05999.

Gómez-Gómez, L.; Bauer, Z.; Boller, T. (2001): Both the extracellular leucine-rich repeat domain and the kinase activity of FSL2 are required for flagellin binding and signaling in Arabidopsis. In: Plant Cell 13 (5), S. 1155–1163. Online verfügbar unter https://pubmed.ncbi.nlm.nih.gov/11340188.

Koller, Teresa; Bent, Andrew F. (2014): FLS2-BAK1 extracellular domain interaction sites required for defense signaling activation. In: PLOS ONE 9 (10), e111185-e111185. DOI: 10.1371/journal.pone.0111185.