Part:BBa_K3610003

BAK1 Ectodomain from Arabidopsis thaliana

This part is a version of the BAK1 receptor (Part:BBa_K3610001 for full length) without the intracellular kinase domain and without the native signal sequence.

Usage and Biology

The BAK1 cell surface receptor from A. thaliana consists of a single pass transmembrane domain and two topoligical domains, an extracellular and a cytoplasmic one. The cytoplasmic domain functions as a kinase domain and initiates a signal cascade upon dimerization with a plant PRR to activate the plant immune response.

For visualizing the interaction of BAK1 with other plant receptors the cytoplasmic domain can be replaced with a split fluorescent protein or another protein that generates a visual output. In our iGEM project, we fused this sequence to a split-mCherry protein and a split-luciferase and then coexpressed the part with another plant PRR carrying the counterpart to the split protein instead of the intracellular kinase domain.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 576
  Illegal PstI site found at 621
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 576
  Illegal PstI site found at 621
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 576
  Illegal PstI site found at 621
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 576
  Illegal PstI site found at 621
  
• 1000
  COMPATIBLE WITH RFC[1000]