Coding

Part:BBa_K3610013:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-09-13)
Revision as of 15:57, 13 September 2020 by Jtrott (Talk | contribs) (→‎Source)


NanoLuc - codon optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 43
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codons with a frequency lower than 10% were considered rare and were replaced for optimal expression. As alternative codons, the ones with the highest frequencies were chosen unless it added unwanted restriction sites and was therefore incompatible with iGEM standards.


Source

The sequence is a modified version of the sequence of Part:BBa_K1159001 from the iGEM registry

References