Part:BBa_K3610013:Design

NanoLuc - codon optimized for C. reinhardtii

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 227
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 43
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Codons with a frequency lower than 10% were considered rare and were replaced for optimal expression. As alternative codons, the ones with the highest frequencies were chosen unless it added unwanted restriction sites and was therefore incompatible with iGEM standards.

Source

The sequence is a modified version of the sequence of Part:BBa_K1159001 from the iGEM registry

References

Barahimipour, Rouhollah; Strenkert, Daniela; Neupert, Juliane; Schroda, Michael; Merchant, Sabeeha S.; Bock, Ralph (2015): Dissecting the contributions of GC content and codon usage to gene expression in the model alga Chlamydomonas reinhardtii. In: Plant J 84 (4), S. 704–717. DOI: 10.1111/tpj.13033.

England, Christopher G.; Ehlerding, Emily B.; Cai, Weibo (2016): NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence. In: Bioconjug Chem 27 (5), S. 1175–1187. DOI: 10.1021/acs.bioconjchem.6b00112.