DNA

Part:BBa_K3693002:Design

Designed by: Ziyu Liu   Group: iGEM20_BJ101HS   (2020-09-12)
Revision as of 09:25, 12 September 2020 by Registry (Talk | contribs)

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coding for κ-casein in Homo sapiens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There are two considerations that we have during the detailed design of this sequence. On the one hand, we acknowledged the differences between humans yeasts,doing codon optimization to this sequence in order to increase protein expression. On the other hand, we put His-tag to the sequence, which indeed helps purify proteins.


Source

atgaaatcttttttgttggttgttaacgctttggctttgaccttgccatttttggctgttgaagttcaaaaccaaaaacaaccagcttgtcacgaaaacgacgaaagacctttttaccaaaagactgctccatacgttccaatgtattacgttccaaactcttacccttactatggtactaatttgtaccagagaagaccagctatcgctattaacaacccatacgttcctagaacctactacgctaacccagctgttgttagaccacatgctcaaattcctcaaagacagtatttgccaaactcccacccaccaaccgttgttagattgccaaacttgcacccatccttcattgctattccaccaaaaaagattcaagataagattattattccaactattaacactattgctactgttgaaccaactccagctccagctactgaaccaactgttgattctgttgttactccagaagccttttctgaatctattattacttctactccagaaactactactgttgctgttactccaccaactgct

References