DNA
Part:BBa_K3693002:Design
Designed by: Ziyu Liu Group: iGEM20_BJ101HS (2020-09-12)
coding for κ-casein in Homo sapiens
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
There are two considerations that we have during the detailed design of this sequence. On the one hand, we acknowledged the differences between humans yeasts,doing codon optimization to this sequence in order to increase protein expression. On the other hand, we delete the stop codon in order to add tag or construct chimera easily.
Source
The original source is from Homo sapiens, but we did codon optimization.