Part:BBa_K733001

Ptms - a constitutive promoter in E. coli and B. subtilis.

Ptms is a constitutive promoter that can be recognized by SigA factor in Bacillus subtilis. Due to E. coli’s promiscuousness in recognizing transcription and translation signals, Ptms is also functional in E. coli. According to some experiments, Ptms shows quite low efficiency of transcription in B. subtilis. ( Moran et al., 1982).

For the characterization of Ptms, please refer to: BBa_K733009

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Functional Parameters: Austin_UTexas

BBa_K733001 parameters

Burden Imposed by this Part:

Burden Value: 6.3 ± 10.6%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.

Reference

Moran, C., Lang, N., LeGrice, S., Lee, G., Stephens, M., Sonenshein, A., et al. (1982). Nucleotide sequences that signal the initiation of transcription and translation inBacillus subtilis..Molecular and General Genetics MGG,186, 339-346.