Part:BBa_B0013

biology	T7TE (+/-)
direction	Forward
forward_efficiency	0.6[CC]
reversed_version	591
reverse_efficiency	-1.06[CC]

TE from coliphage T7 (+/-)

Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.
But notice that the measurement indicates it is a strong promoter in the reverse direction.

Secondary Structure

File:Mfold-B0013-1.png

Measurement

[http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

Functional Parameters: Austin_UTexas

BBa_B0013 parametersK598014K598014

Burden Imposed by this Part:

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.