Part:BBa_P10302

GVG

PhytoBricks part from plasmid pUAP80020

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1230
  Illegal PstI site found at 502
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1230
  Illegal PstI site found at 502
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1230
  Illegal BamHI site found at 482
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1230
  Illegal PstI site found at 502
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1230
  Illegal PstI site found at 502
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1
  Illegal BsaI.rc site found at 1328
  Illegal SapI.rc site found at 1028
  

Functional Parameters: Austin_UTexas

BBa_P10302 parameters

Burden Imposed by this Part:

Burden Value: -0.5 ± 2.1%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.