Regulatory

Part:BBa_K3634018:Design

Designed by: Laurence Seeley   Group: iGEM20_St_Andrews   (2020-08-08)
Revision as of 11:31, 9 August 2020 by Ls268 (Talk | contribs) (→‎Source)


PL8-UV5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The wt sequence for the lac operon regulatory region was taken from Kalnins A. (1993) [GenBank accession no. J01636]. Within the CAP binding site, bases -66 (G) and -55 (C) of the wt binding region were substituted with A and T respectively to prevent binding of the CAP protein at low glucose concentrations. The wt -10 promoter sequence was also mutated from TATGTT to TATAAT in order to allow σ factor (RpoD) to bind without relying on further activation by the CAP protein (Reznikoff et al., 1978).

Source

The lac operon is found natively in E.coli. The part sequence was taken from BBa_K1695000 who generated the part sequence through data obtained from Reznikoff et al. (1978) and Hirschel et al. (1980).

References