Part:BBa_K3634018:Design

PL8-UV5

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The wt sequence for the lac operon regulatory region was taken from Kalnins A. (1993) [GenBank accession no. J01636]. Within the CAP binding site, bases -66 (G) and -55 (C) of the wt binding region were substituted with A and T respectively to prevent binding of the CAP protein at low glucose concentrations. The wt -10 promoter sequence was also mutated from TATGTT to TATAAT in order to allow σ factor (RpoD) to bind without relying on further activation by the CAP protein (Reznikoff et al., 1978).

Source

The lac operon is found natively in E.coli. The part sequence was taken from BBa_K1695000 who generated the part sequence through data obtained from Reznikoff et al. (1978) and Hirschel et al. (1980).

References

Jacob F., Monod J. 1961. Genetic Regulatory Mechanisms in the Synthesis of Proteins. J. Mol. Biol. 3: p818-356.

Reznikoff W. S., Abelson J. N. 1978. The lac promoter. The operon. 7: p221-243. DOI: 10.1101/0.221-243

Hirschel B.J., Shen V., Schlessinger D. 1980. Lactose Operon Transcription from Wild-Type and L8-UV5 lac Promoters in Escherichia coli Treated with Chloramphenicol. J. Bacteriol. 143(3): p1534-1537.

City University of Hong Kong iGEM 2015 - https://parts.igem.org/Part:BBa_K1695000