Regulatory
Part:BBa_K3634018:Design
Designed by: Laurence Seeley Group: iGEM20_St_Andrews (2020-08-08)
PL8-UV5
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Unusually, pre-existing parts on the registry for the ccdA antitoxin had differing sequences. Thus a new twin part was categorised with a different sequence.
Source
The ccdA antitoxin is found natively in E.coli with sequence obtained from the pCoo plasmid (NCBI Accession Number: NC_007635.1). This was confirmed with sequence from NCBI Accession Number: NC_019000.