Part:BBa_K3634008:Design
Phycocyanobilin:Ferredoxin Oxidoreductase (pcyA) (Optimised for E.coli)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence from BBa_I15009 was taken and subject to the IDT codon optimisation tool. During optimisation, no illegal restriction sites were created allowing for the part to be used at RFC[10] and RFC[1000] standard.
Source
The pcyA gene is found in Synechocystis sp. PCC 6803 and has been previously categorised in BioBrick form by J.J. Tabor (BBa_I15009). In order to maximise expression of phycocyanobilin:ferredoxin oxidoreductase in the chosen E.coli chassis, the St Andrews iGEM team of 2020 carried out codon optimisation using the IDT codon optimisation tool allowing for the part to be used at RFC[10] and RFC[1000] standard.
References
UTAustin iGEM 2004 - https://parts.igem.org/Part:BBa_I15009
IDT Codon Optimisation Tool - https://eu.idtdna.com/CodonOpt