Part:BBa_K3634008:Design

Phycocyanobilin:Ferredoxin Oxidoreductase (pcyA) (Optimised for E.coli)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The sequence from BBa_I15009 was taken and subject to the IDT codon optimisation tool. During optimisation, no illegal restriction sites were created allowing for the part to be used at RFC[10] and RFC[1000] standard.

Source

The pcyA gene is found in Synechocystis sp. PCC 6803 and has been previously categorised in BioBrick form by J.J. Tabor (BBa_I15009). In order to maximise expression of phycocyanobilin:ferredoxin oxidoreductase in the chosen E.coli chassis, the St Andrews iGEM team of 2020 carried out codon optimisation using the IDT codon optimisation tool.

References

UTAustin iGEM 2004 - https://parts.igem.org/Part:BBa_I15009

IDT Codon Optimisation Tool - https://eu.idtdna.com/CodonOpt

UniProtKB: Q55891 (PCYA_SYNY3) - https://www.uniprot.org/uniprot/Q55891