Plasmid_Backbone
Eukaryotic

Part:BBa_K157040

Designed by: iGEM Team Freiburg 2008   Group: iGEM08_Freiburg   (2008-10-26)
Revision as of 01:39, 27 October 2008 by Registry (Talk | contribs)

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Eukaryotic terminator vector (very similar to pSB1AK8)

Transfection vector with CMV Promotor

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal XbaI site found at 3420
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 8
    Illegal NotI site found at 3411
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal XhoI site found at 1272
    Illegal XhoI site found at 2298
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3405
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3420
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2444


[edit]
Categories
//chassis/eukaryote
//plasmidbackbone/operation
Parameters
chassis
copies
originpMB1
resistanceAK