Plasmid_Backbone
Eukaryotic

Part:BBa_K157040

Designed by: iGEM Team Freiburg 2008   Group: iGEM08_Freiburg   (2008-10-26)

Eukaryotic terminator vector (very similar to pSB1AK8)

This vector is originally BioBrick vector pSB1AK3 with eukaryotic terminator added. pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

It is derived from BBa_J52017. There are a couple differences. It lacks the 3' G in the BioBrick prefix (which should be present to comply with the assembly standard 10). It is also missing an A in the BioBrick suffix between the SpeI site and NotI site (which also should be present to comply with assembly standard 10).

  • RS 19:26, 23 November 2008 (UTC): This part has been split into a plasmid backbone and part and remapped to BBa_K157040-BBa_K157042. BBa_K157040 is the plasmid backbone and BBa_K157042 is the part. The physical DNA needs to be remapped to BBa_K157042.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal XbaI site found at 3420
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 8
    Illegal NotI site found at 3411
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal XhoI site found at 1272
    Illegal XhoI site found at 2298
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3405
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3420
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2444


[edit]
Categories
//chassis/eukaryote
//plasmidbackbone/operation
Parameters
chassis
copies
originpMB1
resistanceAK