Coding

Part:BBa_K117003:Design

Designed by: Nguyen Xuan Hung   Group: iGEM08_NTU-Singapore   (2008-10-08)
Revision as of 01:32, 27 October 2008 by Lalala8585 (Talk | contribs)

T7ptag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7ptag is responsible for the transcription of T7 RNA polymerase. The polymerase gene has been modified to contain two amber stop codons (T7ptag).

These stop codons are translated as serine when supD is also transcribed. Polymerase is expressed only when both SupD and T7ptag mRNA are present.

The T7ptag was derived from the original AND gate obtained from Voigt lab.

Source

[http://www.voigtlab.ucsf.edu/index.html http://www.voigtlab.ucsf.edu/index.html]

References

Anderson JC, Voigt CA, Arkin AP (2007) [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1964800 Environmental signal integration by a modular AND gate], Molecular Systems Biology, 3 (133)