Part:BBa_K117003:Design

T7ptag

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

T7ptag is responsible for the transcription of T7 RNA polymerase. The polymerase gene has been modified to contain two amber stop codons (T7ptag).

These stop codons are translated as serine when supD is also transcribed. Polymerase is expressed only when both SupD and T7ptag mRNA are present.

The T7ptag was derived from the original AND gate obtained from Voigt lab.

Plasmid pBACr-Mgr940 containing t7ptag derived from plasmid pBAC874t was generously donated by voigt lab. T7ptag is extracted from plasmid pBACr-Mgr940 using PCR with oligo sequences of 5'-CCTAGGAATTAACCGTGACCATGA-3' & 3'-CTGAAGCGCAAGCGTATT-5' with biobricks prefix and suffix respectively.

Source

[http://www.voigtlab.ucsf.edu/index.html http://www.voigtlab.ucsf.edu/index.html]

References

Anderson JC, Voigt CA, Arkin AP (2007) [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1964800 Environmental signal integration by a modular AND gate], Molecular Systems Biology, 3 (133)