Coding

Part:BBa_K3244026

Designed by: Mohammad Tarek Mansour   Group: iGEM19_AFCM-Egypt   (2019-10-10)
Revision as of 21:29, 21 October 2019 by MTarek (Talk | contribs)


Gly Ser Linker for TanCAR-T scFvs

Gly Ser Linker for TanCAR-T scFvs [http://2019.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2019] have worked to create an improved Glycine serine linker in part BBa_K1537017 in our new part BBa_K3244026 to adapt for our bispecific extracellular domain which should carry 2 scFv targeting 2 different antigens. This part has been modified through codon optimization -which has represented an issue for our fragment DNA synthesis due to the need for repetition of linker sequence between 4 different chains of 2 scFvs- and amino acid sequence length editing t(GGGGS)5 to act as an inter-chain linker between the 2 scFv for enhanced production and stability of the bispecific scFv. T2A was also added as a basic part in BBa_K3244020. For reulsults of functional characterization please refer to our parts Page EGYPT-AFCM 2019 Parts

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None