Part:BBa_K3244026

Gly Ser Linker for TanCAR-T scFvs

Gly Ser Linker for TanCAR-T scFvs [http://2019.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2019] have worked to create an improved Glycine serine linker in part BBa_K1537017 in our new part BBa_K3244026 to adapt for our bispecific extracellular domain which should carry 2 scFv targeting 2 different antigens. This part has been modified through codon optimization -which has represented an issue for our fragment DNA synthesis due to the need for repetition of linker sequence between 4 different chains of 2 scFvs- and amino acid sequence length editing t(GGGGS)5 to act as an inter-chain linker between the 2 scFv for enhanced production and stability of the bispecific scFv. T2A was also added as a basic part in BBa_K3244020. For results of functional characterization please refer to our parts Page EGYPT-AFCM 2019 Parts Significant difference between GSSS_ BBa_K1537017 engineered and GSSS_BBa_K3244026 T-cells(p<0.05), higher expression levels were observed in GSSS_BBa_K3244026 group.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]