Regulatory

Part:BBa_K117004:Experience

Designed by: Nguyen Xuan Hung   Group: iGEM08_NTU-Singapore   (2008-10-08)
Revision as of 13:33, 26 October 2008 by ChinChong (Talk | contribs) (→‎Applications of BBa_K117004)

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Abnormality found in the registry

This device could be found in the registry in the form of BBa_J04430. However, the team found an abnormality when BBa_J04430 was transformed into Top 10 cells. As shown on the pictures below, the cells glow pink instead of green under UV light.

BBa_J04430 glows pink


Characterization of pLacI-GFP

The NTU@iGEM team characterized the biobrick part pLacI-GFP. The influence of 1)concentration of Lactose 2) temperature and 3) time were investigated.

Shown below is the graph obtained for the experiment conducted at 37°C.

Characterization at 37°C

Electron microscope pictures

Cells induced with 1mM lactose concentration
Cells induced with 10mM lactose concentration


For more information including the protocols and results obtained, please go to: [http://2008.igem.org/Team:NTU-Singapore/Parts/Characterization_of_LacI-GFP NTU@iGEM Characterization of pLac-GFP]

User Reviews

UNIQf08a2c0909375c7b-partinfo-00000000-QINU UNIQf08a2c0909375c7b-partinfo-00000001-QINU