Part:BBa_K117004:Experience

Abnormality found in the registry

This device could be found in the registry in the form of BBa_J04430. However, the team found an abnormality when BBa_J04430 was transformed into Top 10 cells. As shown on the pictures below, the cells glow pink instead of green under UV light.

BBa_J04430 glows pink

Characterization of pLacI-GFP

The NTU@iGEM team characterized the biobrick part pLacI-GFP. The influence of 1)concentration of Lactose 2) temperature and 3) time were investigated.

The characterization overlook

Shown below is the graph obtained for the experiment conducted at 37°C.

Characterization at 37°C

Electron microscope pictures

Cells induced with 1mM lactose concentration

Cells induced with 10mM lactose concentration

For more information including the protocols and results obtained, please go to: [http://2008.igem.org/Team:NTU-Singapore/Parts/Characterization_of_LacI-GFP NTU@iGEM Characterization of pLac-GFP]

User Reviews

UNIQe502f4b5b0067f70-partinfo-00000000-QINU UNIQe502f4b5b0067f70-partinfo-00000001-QINU