Part:BBa_K3183000

Erythromycin Constitutive Promoter

P-erm is a constitutive promoter which can be used in Lactobacillus reuteri 10023C, and may have uses in other Lactobacillus species. It has also been shown to be functional in E. coli.

The promoter is derived from the erythromycin ribosomal methylase (ermB) promoter from the broad-host range plasmid pAMβ1 isolated from Enterococcus faecalis.¹ It was subsequently characterised in six strains of Lactobacillus reuteri and Lactococcus lactisspp. cremoris MG1363.²

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Use by Team Oxford 2019

This transcription factor is part of our detection system in L. reuteri. The composite parts containing this AgrA2 protein are BBa_K3183102, and BBa_K3183300.

Characterisation

This part was characterised in the composite part BBa_K3183100.

References

1. Swinfield, Tracy-Jane, et al. “Physical Characterisation of the Replication Region of the Streptococcus Faecalis Plasmid pAMβ1.” Gene, vol. 87, no. 1, 1990, pp. 79–90., doi:10.1016/s0378-1119(19)30488-3.
2. Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri  Strains.” FEMS Microbiology Letters, vol. 308, no. 1, 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.