Composite

Part:BBa_K3205000:Design

Designed by: Shengkun Tong   Group: iGEM19_HZAU-China   (2019-10-07)
Revision as of 13:20, 21 October 2019 by TSK (Talk | contribs) (→‎Source)


A device for detection benzyl alcohol by producing GFP.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 66
    Illegal BglII site found at 712
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 673
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2772


Design Notes

There is a stem-loop structure in the downstream Pare manipulation sequence of AreR, so it is not necessary to add additional terminators.

Source

areR and Pare sequences come from are operon of Acinetobacter sp. Strain ADP1.

References

1.Jones R M, Williams P A. areCBA Is an Operon inAcinetobacter sp. Strain ADP1 and Is Controlled by AreR, a ς54-Dependent Regulator[J]. Journal of bacteriology, 2001, 183(1): 405-409.

2.Jones R M, Williams P A. Mutational analysis of the critical bases involved in activation of the AreR-regulated σ54-dependent promoter in Acinetobacter sp. strain ADP1[J]. Appl. Environ. Microbiol., 2003, 69(9): 5627-5635.