Composite
Part:BBa_K3205000:Design
Designed by: Shengkun Tong Group: iGEM19_HZAU-China (2019-10-07)
A device for detection benzyl alcohol by producing GFP.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 66
Illegal BglII site found at 712 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 673
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2772
Design Notes
There is a stem-loop structure in the downstream Pare manipulation sequence of AreR, so it is not necessary to add additional terminators.
Source
areR and Pare sequences come from are operon of Acinetobacter sp. Strain ADP1.
References
1.Jones R M, Williams P A. areCBA Is an Operon in Acinetobacter sp. Strain ADP1 and Is Controlled by AreR, a ς54-Dependent Regulator[J]. Journal of bacteriology, 2001, 183(1): 405-409.
2.Jones R M, Williams P A. Mutational analysis of the critical bases involved in activation of the AreR-regulated σ54-dependent promoter in Acinetobacter sp. strain ADP1[J]. Appl. Environ. Microbiol., 2003, 69(9): 5627-5635.