Composite

Part:BBa_K3110007

Designed by: Mrugank Dake, Amartya Pal   Group: iGEM19_IISER_Tirupati   (2019-10-14)
Revision as of 06:32, 21 October 2019 by Suryazzz (Talk | contribs)


Medium Promoter Strong RBS lldD

This construct has L-Lactate Dehydrogenase (lldD) under the control of a medium promoter and a strong RBS. lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD by varying the strength of the promoter and RBS controlling its production.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1202
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 639


Characterization of the Part

Figure 1. Relative Expression of sfGFP under the control of lldRO1-J23117-lldRO2(Part:BBa_K1847008)-strong RBS and Constitutive Promoter-strong RBS along with Medium Promoter-Strong RBS-lldD.

Comparison between only O1PO2 and O1PO2 along with lldD

[[File:T--IISER Tirupati--compare.png|thumb|center|700px|Figure 1. Relative Expression of sfGFP under the control of only lldRO1-J23117-lldRO2(Part:BBa_K1847008)-strong RBS and lldRO1-J23117-lldRO2-strong RBS along with Medium Promoter-Strong RBS-lldD (BBa_K3110007).

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Parameters
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