Device
TARS-LacI

Part:BBa_K108030:Design

Designed by: Shan Lin   Group: iGEM08_Tsinghua   (2008-10-23)
Revision as of 15:16, 24 October 2008 by Angel6767Q (Talk | contribs) (→‎Design Notes)

TARS-LacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 31


Design Notes

LacI Operator sequence was designed according to reference 1.

TARS sequence was designed according to reference 2.

LVA sequence was designed according to reference 3.

PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.

Source

LacI and T7 terminator was cloned from pET-15b(Novagen).

References

1. Bacterial Transcriptional Regulators for Degradation Pathways of Aromatic Compounds. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS. 2004, 68(3):474–500.

2. An Artifical Aptazyme-Based Riboswitch and its Cascading System in E.coli. ChemBioChem. 2008, 9:206-209.

3. New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 1998, 64(6):2240–2246.